Table 1.

Summary of Recommended Plasmids Usage for Different Applications of CRSIPR-dCas13b on Living Cell Imaging

Assay	Plasmids Usage
Single RNA labeling and tracking	0.3 μg dPspCas13b-FP, 0.7 μg gRNA (Psp)
Dual-color imaging of different RNA	0.3 μg dPspCas13b-FP, 0.7 μg gRNA (Psp), 0.3 μg dPguCas13b-FP, 0.7μg gRNA (Pgu)
Dual-color imaging of genomic DNA and RNA	0.3 μg dPspCas13b-FP, 0.7 μg gRNA (Psp), 0.3 μg dCas9-FP, 0.7 μg sgRNA