REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins
Lipofectamine 3000 Reagent	Thermo	Cat#: 21341
DPBS	Gibco	Cat#: 14190136
FluoroBrite™ DMEM	Thermo	Cat#: A1896701
Dulbecco’s Modified Eagle medium (DMEM)	Gibco	Cat#: 11965118
Fetal Bovine Serum (FBS)	Gibco	Cat#: 10099141
Opti-MEM	Gibco	Cat#: 31985070
Experimental Models: Cell Lines
HeLa	ATCC	Cat#: CCL-2
Recombinant DNA
pCMV-VSV-G	Addgene	Cat#: 8454
pMD2.G	Addgene	Cat#: 12259
PspCas13b crRNA backbone	Addgene	Cat#: 103854
PguCas13b crRNA backbone	Addgene	Cat#: 103853
dCas9 sgRNA backbone	Addgene	Cat#: 75390
pHAGE-dPspCas13b-3×EGFP-2×NLS-IRES-puro	Addgene	Cat#: 132397
pHAGE-dPspCas13b-3×sfGFP-3×NLS-IRES-puro	This paper	N/A
pHAGE-dPspCas13b-2×mNeonGreen-NLS-IRES-puro	Addgene	Cat#: 132403
pHAGE-dPspCas13b-3×mRuby3-2×NLS	This paper	N/A
pHAGE-dPguCas13b-3×mRuby3-2×NLS	This paper	N/A
pHAGE-TO-dCas9-mEmerald	This paper	N/A
Software and Algorithms
Fiji/ImageJ	Fiji/ImageJ	https://imagej.net/Fiji/
GraphPad Prism 8	GraphPad Software	http://www.graphpad.com/scientific-software/prism/
gRNAs Design Tool	CRISPR DESIGN	http://crispor.tefor.net
Office365	Microsoft	https://www.office.com
MATLAB	MathWorks	https://www.mathworks.com
softWoRx 6.5	GE Healthcare	N/A
Other
DeltaVision Elite imaging system	GE Healthcare	N/A
35 mm no.1.5 glass-bottomed dishes	Cellvis	Cat#: D35-2001.5-N