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. 2020 Jun 3;1(1):100039. doi: 10.1016/j.xpro.2020.100039

Figure 1.

Figure 1

Bioinformatic Analysis for the Presence of Native CRISPR-Cas Locus and Its Spacers, Repeat, and PAM Sequences

(A) Output results showing the presence of a type I-F CRISPR-Cas system by CRISPRCasFinder search following input of PA154197 genome sequence as the query. Blast of the identified CRISPR arrays and cas genes against the genome of PA154197 led to assembly of the complete native CRISPR-Cas loci in the strain. Diamonds and rectangles indicate the repeats and spacers in the CRISPR arrays, respectively. Number of spacers is indicated above each of the CRISPR arrays. Bended arrows (black) above the leader sequence (purple) indicate the orientation of CRISPR transcription.

(B) Alignment of 8-bp upstream sequences of protospacers identified from the bacteriophage and plasmid databases led to prediction of the PAM sequence 5′-CC-3′ (Blue). A frequency chart is plotted to visualize the PAM sequence by inputting all the available PAM-Protospacer sequences using WebLogo.