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. 2020 Jun 3;1(1):100039. doi: 10.1016/j.xpro.2020.100039

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© 2020 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Sequence Information of the Platform Plasmid pAY5211 and the pEditing pAY5235 Constructed for mexB Deletion

(A) pAY5211 contains a kanamycin-resistant gene and a luciferase-encoding operon (luxCDABE) for the dual selection of transformants. Mini-CRISPR is inserted between KpnI and BamHI which is co-transcribed with the luxCDABE operon driven by the Ptat promoter. Donor fragments are assembled at the XhoI site. Primers pMS402-F/R are used to verify the constructs of pTargeting and pEditing.

(B) Sequence information of the mini-CRISPR and donor in pAY5235 employed to construct mexB deletion is shown.