| Cycle Step | Temperature (°C) | Time | Number of Cycle |
|---|---|---|---|
| Initial Denaturation | 98 | 5 min | 1 |
| Denaturation | 98 | 30 s | ×35 |
| Annealing | 62 | 30 s | |
| Extension | 72 | 10 s | |
| Final Extension | 72 | 10 min | 1 |
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