| Component | Volume (μL) |
|---|---|
| 10×CutSmart Buffer | 5 |
| pAY5211/ PCR product from Step 2 | X μL (12.5 μg/5 μg) |
| KpnI | 2 |
| BamHI | 2 |
| Milli-Q H2O | to final 50 |
| Total Volume | 50 |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.