| Component | Volume (μL) |
|---|---|
| 2×Quick Ligation Reaction Buffer | 10 |
| Digested pAY5211 | X μL (0.5 μg) |
| Digested mini-CRISPR | X μL (0.1 μg) |
| Quick T4 DNA Ligase | 1 |
| Milli-Q H2O | to final 20 |
| Total Volume | 20 |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.