| Component | Volume (μL) |
|---|---|
| 10×Taq buffer | 1 |
| 10 mM dNTP mix | 0.2 |
| Primer 1 (pMS402-F, 10 μM) | 1 |
| Primer 2 (pMS402-R, 10 μM) | 1 |
| DNA template (Colony suspension in 10 μL MilliQ H2O) | 0.2 |
| Taq DNA Polymerase | 1 |
| Milli-Q H2O | 5.6 |
| Total Volume | 10 |
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