REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Strains
PA154197	This study	N/A
PA150567	This study	N/A
Ocean-100	Kumagai et al., 2017	N/A
DH5α	This study	N/A
Chemicals, Peptides, and Recombinant Proteins
Kanamycin disulfate	Sigma-Aldrich	Cat#K1876
KpnI	New England Biolabs	Cat#R3142
BamHI	New England Biolabs	Cat#R3136
XhoI	New England Biolabs	Cat#R0146
Critical Commercial Assays
Quick Ligation™ Kit	New England Biolabs	Cat#M2200
ClonExpress II One Step Cloning Kit	Vazyme	C112
iProof™ High-Fidelity DNA Polymerase	Bio-Rad	Cat#1725301
Taq DNA polymerase	Thermo Scientific	Cat#EP0406
illustra GFX PCR DNA and Gel Band Purification Kit	GE Healthcare	28-9034-70
MiniBEST Agarose Gel DNA Extraction Kit	Takara	Cat#9761
QIAprepTM Spin Miniprep Kit	Qiagen	Cat#27104
Oligonucleotides
pUC57-F: 5′-AGGGTTTTCCCAGTCACGAC-3′	This study	N/A
pUC57-R: 5′-AGCGGATAACAATTTCACAC-3′	This study	N/A
pMS402-F: 5′-CCAGCTGGCAATTCCGA-3′	This study	N/A
pMS402- R: 5′-AATCATCACTTTCGGGAAAG-3′	This study	N/A
Mini-CRISPR: 5′-GTTCACTGCCGTATAG GCAGCTAAGAAANNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNGTTC ACTGCCGTATAGGCAGCTAAGAAA-3′	This study	N/A
Donor-UF: 5′-CCCTTTCGTCTTCACCT CGAGNNNNNNNNNNNNN NNNNNNN-3′	This study	N/A
Donor-DR: 5′-GATCAGGAACACCCCC TCGAGNNNNNNNNNNNNNNNNN NNN-3′	This study	N/A
Recombinant DNA
pAY5211	This study	N/A