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The Design of the Lambda Phage Genome within the Yeast Centromere Plasmid, pRS415
(A) The full lambda phage genome in genomic orientation from attL to attR is inserted in pRS415 after the promoter for the LEU2 gene.
(B) The lambda phage genome through attR, with 72 bp downstream in the E. coli genome is inserted right before the S. cerevisiae centromere sequence.
(C) The lambda phage genome from attL, including 57 bp upstream, is inserted after the LEU2 promoter.
