Table 2.
Primer Sets Used in This Study
| Primer | Sequence | Purpose |
|---|---|---|
| KB135 | Tataacgtttttgaacacacatgaacaaggactgaaaatgtgttcacaggttgctccggg | Amplify insert 1 |
| KB137 | gggaaagataagcgctcaataaacctgtctg | Amplify insert 1 |
| KB253 | tgatgattatcagccagcagagaattaagg | Amplify insert 2 |
| KB258 | agactgctttgatgtgcaaccgacgacgac | Amplify insert 2 |
| KB256 | gctgcgctcgatgcaaaatacacgaaggag | Amplify insert 3 |
| KB254 | tttcctcaccgatggtcagcgtgtctccac | Amplify insert 3 |
| KB257 | ctccagcccgtccctgtttgtccggactga | Amplify insert 4 |
| KB259 | atattgatactggcggctatccagtacagc | Amplify insert 4 |
| KB255 | attgcggatatcagacaggttgaaaccagc | Amplify insert 5 |
| KB47 | gggtattgtttgttcccctgagcgcggttg | Amplify insert 5 |
| KB48 | caaccgcgctcaggggaacaaacaatacccaaagaggagaaatactagatgtcgg | Amplify insert 6 |
| KB51 | cggccagtacaaaagcggtgttcgcaatctttagcggtgacccagttttg | Amplify insert 6 |
| KB50 | agattgcgaacaccgcttttgtactggccg | Amplify insert 7 |
| KB136 | acattcaaatatgtatccgctcatgagacaggcgcaatgccatctggtatcacttaaagg | Amplify insert 7 |
| KB158 | tgtctcatgagcggatacatatttgaatgt | Amplify YAC |
| KB159 | ccttgttcatgtgtgttcaaaaacgttata | Amplify YAC |
| KB162 | agcgcccctgtgtgttctcgttatgttgag | Validate insert YAC-1 junction |
| KB163 | aagcatcaggtctttccttcgaaggggatc | Validate insert YAC-1 junction |
| KB164 | aacgcgctctccactgcttaatgacattcc | Validate insert 1–2 junction |
| KB165 | aaagttatcgctagtcagtggcctgaagag | Validate insert 1–2 junction |
| KB166 | gcggcaattactgacatgcagatgcgtcag | Validate insert 2–3 junction |
| KB167 | cctgattgcccgacattatcg | Validate insert 2–3 junction |
| KB168 | accgtgattctggatacgtctgaactggtc | Validate insert 3–4 junction |
| KB169 | aagccagagatgacaacttccgccatcatc | Validate insert 3–4 junction |
| KB170 | tgagtttcctgctccgtctgaccgtaacag | Validate insert 4–5 junction |
| KB171 | cactctttcgaaaactcctccagtctgctg | Validate insert 4–5 junction |
| KB58 | ggatgaaacgaacagaaaagcccac | Validate 5–6–7 junction |
| KB62 | ctgcggccagttcattcag | Validate 5–6–7 junction |
| KB172 | cctttaagtgataccagatggcattgcgcc | Validate 7-YAC junction |
| KB173 | cttgcctgtaacttacacgcgcctcgtatc | Validate 7-YAC junction |
| KB302 | ctctggagtgcgacaggttt | binds in phage, amplifies across attR site, check for phage integration |
| KB303 | ccttcccgtttcgctcaagt | binds in E. coli, amplifies across attR site , check for phage integration |