Table 3.

PCR Reactions for Lambda and YAC Fragments

Fragment	Template	Forward Primer	Reverse Primers	Melting Temperature	Expected Size / Extension Time
1	BW25113/lambda WT (gDNA, 50 ng)	KB135	KB137	72°C	9,442 bp/5 min
2	BW25113/lambda WT (gDNA, 50 ng)	KB253	KB258	72°C	9,253 bp/5 min
3	BW25113/lambda Δbor::kan gDNA, 50 ng)	KB256	KB254	72°C	9,637 bp/5 min
4	BW25113/lambda WT (gDNA, 50 ng)	KB257	KB259	72°C	10,050 bp/5 min
5	BW25113/lambda WT (gDNA, 50 ng)	KB255	KB47	72°C	3,143 bp/1 min, 35 s
6	Plasmid containing mKate2 (pPE005), 10 ng	KB48	KB51	64°C	774 bp/30 s
7	BW25113/lambda WT (gDNA, 50 ng)	KB50	KB136	72°C	6,257 bp/3 min, 15 s
YAC	pRS415, 10 ng	KB158	KB159	68°C	2,971 bp/  1 min