Table 4.

PCR Recipe for Phage Fragment PCRs

Reagent	Final Concentration (mM or μM)	Volume (μL)
Template DNA	50 ng gDNA or 10 ng plasmid DNA	Variable
Forward Primer (10 μM)	0.5 μM	2.5
Reverse Primer (10 μM)	0.5 μM	2.5
dNTPs (10 mM)	0.2 mM	1
Phusion HF Polymerase	1 unit / 50 μL PCR	0.5
5× Phusion HF or GC Buffer	1×	10
DMSO	up to 5%	up to 2.5
ddH2O	n/a	to 50
Total	n/a	50