Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
PCR Recipe for Junction Verification Colony PCR
| Reagent | Final Concentration (mM or μM) | Volume (μL) |
|---|---|---|
| Template DNA (bacterial colony resuspension in water or yeast colony resuspension in NaOH) | n/a | 1 |
| Forward Primer (10 μM) | 0.5 μM | 1 |
| Reverse Primer (10 μM) | 0.5 μM | 1 |
| GoTaq Green Master Mix 2× | 1× | 10 |
| ddH2O | n/a | 7 |
| Total | n/a | 20 |
