Table 7.

Thermocycler PCR Protocol for GoTaq Polymerase

PCR Cycling Conditions
Steps	Temperature	Time	Cycles
Initial Denaturation	95°C	5 min	1
Denaturation	95°C	30 s	35 cycles
Annealing	55°C	30 s
Extension	72°C	1 min/kb for genomic DNA
Final Extension	72°C	5 min	1
Hold	4°C	Forever