Reagent	Final Concentration	Amount
NaCl	100 mM	5.8 g
MgSO4∗7H20	8 mM	2 g
1M Tris pH=7.5	50 mM	50 mL
2% w/v gelatin from porcine skin, Type A	0.01%	5 mL
ddH2O	n/a	up to 1,000 mL