| Reagent | Final Concentration | Amount |
|---|---|---|
| DMEM | n/a | 440 mL |
| FBS | 10% | 50 mL |
| 200 mM L-glutamine | 2 mM | 5 mL |
| 100× Penicillin/Streptomycin | 1× | 5 mL |
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