REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Strains
Escherichia coli strain MG1655	Coli Genetic Stock Center	CGSC#7740
Escherichia coli strain BW25113	Baba et al., 2006	N/A
Escherichia coli strain MG1655/PE003	Bodner et al., 2020	N/A
Escherichia coli strain BW25113/PE003	Bodner et al., 2020	N/A
Escherichia coli strain MG1655 attB::[λ-mKate cI (WT)]/ PE003	Bodner et al., 2020	N/A
Escherichia coli strain BW25113 attB::[λ-mKate cI (WT)]/ PE003	Bodner et al., 2020	N/A
Escherichia coli strain MG1655/pUC19	Bodner et al., 2020	N/A
Bacteriophage λ	ATCC	Cat#23724-B2
Bacteriophage λ Δbor::kan cI857	Gift from the Lanying Zeng Lab (TAMU)	N/A
Bacteriophage λ-mKate: cI (WT) Δbor::kan, RBS-mKate2, Δ[ea31,ea59]::PGK-mClover-bgh terminator	Bodner et al., 2020	N/A
E. coli MegaX DH10B T1R Electrocomp Cells	Thermo Fisher Scientific	Cat# C6400-03
E. coli MegaX DH10B attB::[λ-mKate cI (WT)	Bodner et al., 2020	N/A
Chemicals, Peptides, and Recombinant Proteins
FluoroBrite™ DMEM	Life Technologies	Cat#A1896702
DMEM	ThermoFisher Scientific	Cat#11965-118
FBS	Omega Scientific	Cat#FB-12
L-glutamine	Life Technologies	Cat#25030081
Penicillin - Streptomycin (100×)	Life Technologies	Cat#15140-122
HEPES pH=7.0	Sigma	Cat#H0887-100ML
Fibronectin	Sigma-Aldrich	Cat#F0895-5MG
Hygromycin B	Thermo Fisher Scientific	Cat#10687010
Gentamicin	Thermo Fisher Scientific	Cat#15710064
Norfloxacin	Sigma-Aldrich	Cat#N9890-5G
gelatin from porcine skin, Type A	Sigma-Aldrich	Cat#G1890-100G; Cas#9000-70-8
Accutase ®	Sigma-Aldrich	Cat#A6964-100ML
Phusion High-Fidelity DNA Polymerase	New England Biolabs	Cat# M0530S
GoTaq® Green Mastermix	Promega	Cat#M7122
RNase A	Qiagen	Cat#19101
DpnI	New England Biolabs	Cat#R0176S
Difco™ M9 Minimal Salts, 5×	BD	Cat#248510
Minimal SD Base	Takara Bio	Cat#630411
DO Supplement -His/-Leu/-Ura	Takara Bio	Cat#630425
L-Histidine	Sigma	Cat#H8000-25G
Uracil	Sigma	Cat#U0750-25G
YPD Broth	Fisher Scientific (RPI)	Cat#50-489-171
PEG3350 (Polyethylene glycol average Mn 3,350, powder)	Sigma-Aldrich	Cat#202444-250G
Carbenicillin (Disodium Salt)	Fisher Scientific	Cat#BP26485
Kanamycin Monosulfate	Fisher Scientific	Cat#K00475G
Chloramphenicol	Sigma-Aldrich	Cat#C0378-25G
Maltose Monohydrate	EMD Millipore	Cat#MX0160-1
Sheared Salmon Sperm DNA	Invitrogen	Cat# AM9680
Sodium Hydroxide	Sigma	Cat#S5881-500G
Luria Agar Granulated [Miller’s LB Agar]	RPI	• Cat#L24022-1000
LB Broth (Miller) Mix	Genesee Scientific	Cat#11-118
BD Bacto™ Dehydrated Agar	Thermo Fisher Scientific	Cat#DF0140-01-0
Agarose (Low-EEO/Multi-Purpose/Molecular Biology Grade)	Fisher Scientific	Cat#BP160-100
D-(+)-Glucose	Sigma	Cat#G8270
Magnesium Sulfate	Sigma-Aldrich	Cat#M2643-500G
Calcium Chloride Dihydrate	EMD Millipore	Cat#1023820250
BD Bacto™ tryptone	FPI Fisher Scientific	Cat#50-213-717
Sodium Chloride	Fisher Scientific	Cat#S271-1
UltraPure™ 1 M Tris-HCl Buffer pH=7.5	Life Technologies	Cat# 15567-027
Critical Commercial Assays
DNeasy Blood and Tissue Kit	Qiagen	Cat#69504
YeaStar™ Genomic DNA Kit	Zymo Research	Cat# D2002
QIAquick PCR Purification Kit	Qiagen	Cat# 28106
Experimental Models: Cell Lines
Mouse: RAW264.7 cells (male)	ATCC	Cat#TIB-71; RRID:CVCL_0493
Mouse: RAW264.7 cells (male), pGK-HygroR,pGK-H2B-miRFP670	Bodner et al., 2020	N/A
Experimental Models: Organisms/Strains
Saccharomyces cerevisiae strain BY4741, MATa his3Δ1 leu2Δ met15Δ ura3Δ	Gift from the Onn Brandman Lab (Stanford)	N/A
Saccharomyces cerevisiae strain BY4741, MATa his3Δ1 leu2Δ met15Δ ura3Δ / pRS415-λ-mKate	Bodner et al., 2020	N/A
Oligonucleotides
Primers for recombinant phage construction and validation and Keio strain validation, see Table 2	Bodner et al., 2020	N/A
Recombinant DNA
Plasmid PE003: SC101 (CmR) - J23119-B0030-mCerulean3 (constitutive bacterial mCerulean expression plasmid)	Bodner et al., 2020	N/A
Plasmid pRS415 (yeast centromere plasmid for expression of LEU2, with bacterial origin of replication), (AmpR)- LEU2, CEN/ARS (S. cerevisiae centromere CEN6 fused to replicating sequence)	Gift from Erin Schwartz (Stanford)	N/A
Plasmid pUC19	Thermo Fisher Scientific	Cat#SD0061
Plasmid PE005: p15A: pL-mKate-LVA	Bodner et al., 2020	N/A
Software and Algorithms
Micro-Manager 2.0	Edelstein et al., 2014	https://micro-manager.org/wiki/Version_2.0
Benchling	Benchling [Biology Software]. (2020). Retrieved from https://benchling.com.	https://benchling.com/
Other
Nunc™ Microwell™ 96-Well Optical-Bottom Plates with Coverglass Base	Thermo Fisher Scientific	Cat#164588
AeraSeal™ film	Sigma-Aldrich	Cat#A9224
Fisherbrand™ Electroporation Cuvettes Plus	Fisher Scientific	Cat# FB101
Thermo Scientific™ Nunc™ Lab-Tek™ Chambered Coverglass	Fisher Scientific	Cat#12-565-472
CELLSTAR® Tissue Culture Plates, Greiner Bio-One	VWR	Cat#655161
Petri dish 100 × 15 mm	Fisher Scientific	Cat# FB0875713
Eclipse Ti fluorescence microscope	Nikon	Ti-E