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. 2020 Jul 15;1(2):100065. doi: 10.1016/j.xpro.2020.100065

Figure 2.

Figure 2

Characterization of Developing vmDA Progenitors and Neurons with 2.5 μM CHIR99021

(A) Immunofluorescence analysis of developing vmDA progenitors differentiated from either H9:PITX3-eGFP or RM3.5:PITX3-eGFP using 2.5 μM CHIR99021. At day 13, vmDA progenitors should homogenously express OTX2, FOXA2 and Nestin, indicating correct midbrain floorplate lineage acquisition.

(B) At day 25, vmDA neurons expressing FOXA2, PITX3, and TH should be readily observed if differentiation has been successful. Scale bar, 100 μm.