REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal anti-BARHLl1	Novus Biologicals	NBP1-86513, RRID:AB_11034569
Mouse monoclonal anti-EN1	DSHB	4G11, RRID:AB_528219
Goat polyclonal anti-FOXA2	Santa Cruz Biotechnology	sc-6554, RRID:AB_2262810
Chicken polyclonal anti-GFP	Abcam	ab13970, RRID:AB_300798
Rabbit polyclonal anti-LMX1A	Millipore	AB10533, RRID:AB_10805970
Rabbit polyclonal anti-NURR1/NUR77	Santa Cruz Biotechnology	Sc-990, RRID:AB_2298676
Goat polyclonal anti-OTX2	R&D Systems	AF1979, RRID:AB_2157172
Sheep polyclonal anti-PITX2	R&D Systems	AF7388, RRID:AB_11128639
Rabbit polyclonal anti-TH	Pel-Freez Biologicals	P40101-0, RRID:AB_461064
Sheep polyclonal anti-TH	Pel-Freez Biologicals	P60101-0, RRID:AB_461070
Chemicals, Peptides, and Recombinant Proteins
Accutase®	Innovative Cell Technologies	AT104
Ascorbic acid	Sigma-Aldrich	A4403
B27™ Supplement or CTS™ B27™ Supplement	Gibco	17504044 A1486701
B27™ Supplement -vitA or CTS™ B27™ Supplement -vitA	Gibco	12587010 A3353501
Brain-derived neurotrophic factor	R&D Systems	248-BDB
CHIR99021	Miltenyi Biotech	130-103-926
Dibutyryl cAMP	Tocris Bioscience	1141
DMEM/F-12 or CTS™ DMEM/F-12	Gibco	11320033 A1370801
Dimethyl sulfoxide	Sigma-Aldrich	D2650
Glial cell line-derived neurotrophic factor	R&D Systems	212-GD
GlutaMax™ Supplement	Gibco	35050061
Insulin-Transferrin-Selenium-Sodium Pyruvate (ITS-A)	Gibco	51300044
KnockOut™ serum replacement or CTS™ KnockOut™ serum replacement	Gibco	10828010 12618012
Laminin-521	BioLamina	LN521
Laminin - mouse	Sigma-Aldrich	L2020
LDN193189	Miltenyi Biotech	130-103-925
MEM Non-Essential Amino Acids	Gibco	11140035
mTeSR™1 or TeSR™2	STEMCELL Technologies	85880 05860
N2 Supplement or CTS™ N2 Supplement	Gibco	17502048 A1370701
N-[N-(3,5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (DAPT)	Sigma-Aldrich	D5942
Neurobasal™ or CTS™ Neurobasal™	Gibco	A3582901 A1371201
PBS+/+ (with Calcium/Magnesium)	Gibco	13492609
PBS−/−	Gibco	14190144
Pierce™ 16% formaldehyde	Thermo Fisher	28906
Purmorphamine	Miltenyi Biotech	130-104-465
ReLeSR™	STEMCELL Technologies	05873
SB431542	R&D Systems	1614
SHH C25II	R&D Systems	464-SH
TGFβ3	Peprotech	100-36E
Trypan blue	Sigma-Aldrich	T8154
Y27632 (ROCK inhibitor) or Y27632-GMP	Tocris Bioscience	1254 TB1254-GMP
Critical Commercial Assays
MycoAlert™ Mycoplasma Detection Kit	Lonza	LT07-218
Experimental Models: Cell Lines
H9 PITX3-eGFP hESC	Colin Pouton Laboratory. (Watmuff et al., 2015)	N/A
RM3.5 PITX3-eGFP hiPSC	In house	N/A
Other
48-well plate – Nunclon Delta surface	Thermo Fisher	150687
Bright-Line™ hemocytometer	Sigma-Aldrich	Z359629
Cryovials – 1.5 mL	Thermo Fisher	366656
Mr. Frosty™ freezing container	Thermo Fisher	5100-0001
T25 Flask	Corning	353108