REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-mouse CD4-PerCP-Cy5.5 (clone: RM4-5)	TONBO Biosciences	Cat# 65-0042-U100; RRID: AB_2621876
Anti-mouse CD8a-PerCP-Cy5.5 (clone: 53-6.7)	TONBO Biosciences	Cat# 65-0081-U100; RRID: AB_2621882
Anti-mouse B220-PerCP-Cy5.5 (clone: RA3-6B2)	TONBO Biosciences	Cat# 65-0452-U100; RRID: AB_2621892
Anti-mouse B220-APC (clone: RA3-6B2)	BioLegend	Cat# 103212; RRID: AB_312997
Anti-mouse Ter-119-PerCP-Cy5.5 (clone: TER-119)	TONBO Biosciences	Cat# 65-5921-U100
Anti-mouse Gr1 (Ly-6G/6C)-PerCP-Cy5.5 (clone: RB6-8C5)	BioLegend	Cat# 108428; RRID: AB_893558
Anti-mouse Gr1-PE-Cy7 (clone: RB6-8C5)	TONBO Biosciences	Cat# 60-5931-U100; RRID: AB_2621870
Anti-mouse Mac1 (CD11b)-PerCP-Cy5.5 (clone: M1/70)	TONBO Biosciences	Cat# 65-0112-U100; RRID: AB_2621885
Anti-mouse Sca-1 (Ly-6A/E)-PE-Cy7 (clone: E13-161.7)	BioLegend	Cat# 122514; RRID: AB_756199
Anti-mouse c-Kit (CD117)-APC-Cy7 (clone: 2B8)	BioLegend	Cat# 105826; RRID: AB_1626278
c-Kit (CD117) MicroBeads Mouse	Miltenyi Biotec	Cat# 130-091-224
Anti-mouse CD150-PE (clone: TC15-12F12.2)	BioLegend	Cat# 115904; RRID: AB_313683
Anti-mouse CD150-BV421 (clone: TC15-12F12.2)	BioLegend	Cat# 115926; RRID: AB_2562190
Anti-mouse CD48-FITC (clone: HM48-1)	BioLegend	Cat# 103404; RRID: AB_313019
Anti-mouse CD48-PE (clone: HM48-1)	BioLegend	Cat# 103406; RRID: AB_313021
Anti-mouse CD41-FITC (clone: MWReg30)	BD Biosciences	Cat# 553848; RRID: AB_395085
Anti-mouse CD41-PE (clone: MWReg30)	BD Biosciences	Cat# 558040; RRID: AB_397004
Anti-mouse CD41-APC (clone: MWReg30)	BioLegend	Cat# 133914; RRID: AB_11125581
Anti-P-selectin (CD62P)-BV421 (clone: RB40.34)	BD Biosciences	Cat# 564289
Anti-mouse EPCR (CD201)-PE (clone: RCR-16)	BioLegend	Cat#141504
Anti-CD34-BV421 (clone: RAM34)	BD Biosciences	Cat# 562608; RRID: AB_11154576
Anti-Flt3 (CD135)-APC (clone: A2F10)	BioLegend	Cat# 135310; RRID: AB_2107050
Anti-mouse CD45-PE-Cy7 (clone: 30-F11)	BioLegend	Cat# 103114; RRID: AB_312979
Anti-mouse CD45-APC-Cy7 (clone: 30-F11)	BD Biosciences	Cat# 557659; RRID: AB_396774
Anti-mouse CD45-FITC (clone: 30-F11)	BD Biosciences	Cat# 553080; RRID: AB_394610
Anti-mouse CD45-PE (clone: 30-F11)	BD Biosciences	Cat# 553081; RRID: AB_394611
Anti-mouse CD45-BV421 (clone: 30-F11)	BioLegend	Cat# 103133; RRID: AB_10899570
Anti-mouse CD45-APC (clone: 30-F11)	BD Biosciences	Cat# 559864; RRID: AB_398672
Anti-mouse CD45-PerCP-Cy5.5 (clone: 30-F11)	BD Biosciences	Cat# 550994; RRID: AB_394003
Fc-block (anti-mouse CD16/32) (clone: 2.4-G2)	BD Biosciences	Cat# 553142; RRID: AB_394657
Chemicals, Peptides, and Recombinant Proteins
PBS	Nacalai Tesque	Cat# 14249-24
Fetal bovine serum	Thermo Fisher Scientific	Cat# 26140079
DMEM/F-12 medium	Thermo Fisher Scientific	Cat# 11320-033
IST	Thermo Fisher Scientific	Cat# 41400-045
Penicillin	Meiji Seika	PGLD755
Streptomycin sulfate	Meiji Seika	SSDN1013
Sodium selenite	Nacalai Tesque	Cat# 11707-04
Bovine serum albumin	Sigma Aldrich	Cat# A4503
Fatty-acid free bovine serum albumin	Sigma Aldrich	Cat# A7030-10G
2-Mercaptoethanol (2-ME) 1000×	Life Technologies	Cat# 21985-023
Sodium palmitate	Tokyo Chemical Industry Co., Ltd.	Cat# P0007
Sodium oleate	Tokyo Chemical Industry Co., Ltd.	Cat# O0057
Cholesterol	Tokyo Chemical Industry Co., Ltd.	Cat# C0318
Ammonium Chloride	Fujifilm	Cat# 017-2995
Sodium Hydrogen Carbonate	Fujifilm	Cat# 191-01305
EDTA 2Na	Fujifilm	Cat# 345-01865
Recombinant Murine SCF	PeproTech	Cat# 250-03
Recombinant Human TPO	PeproTech	Cat# 300-18
Recombinant Murine Flt3 ligand	PeproTech	Cat# 250-31L
Recombinant Murine IL6	PeproTech	Cat# 216-16
Recombinant human Flt3 ligand	PeproTech	Cat# 300-19
Recombinant human insulin	Nacalai Tesque	Cat# 12878-86
Recombinant human holo-transferrin	Nacalai Tesque	Cat# 34443-44
Propidium iodide	Life Technologies	Cat# P3566
Flow-Check Fluorospheres	Beckman Coulter	Cat# 7547053
Experimental Models: Organisms/Strains
Mouse: C57BL/6JJmsSlc	Japan SLC, Inc.	http://www.jslc.co.jp/english/index2.htm
Software and Algorithms
FlowJo version 10	BD Biosciences	https://www.flowjo.com/solutions/flowjo
Other
AutoMACS Pro	Miltenyi Biotec	N/A
FACS Aria3u	BD Biosciences	N/A
VELVO-CLEAR VS-25 (sonicator)	VELVO-CLEAR	N/A