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LEC Sorting from Human LNs for Single-Cell Analysis
Gating strategy for isolation of LECs from human LNs. CD45+ cells and dead cells are excluded based on CD45 and Live/Dead dead cell stain, respectively. Live single CD45-PDPN+CD31+ are sorted for the single-cell analysis of human LECs. Adopt from Figure S1A of Takeda et al., 2019.
