Viability of Dissociated Human LN Stromal Cell Subsets after Cell Dissociation and Cell Sorting
(A and B) Viability of CD45+ cells, SCs, BECs and LECs dissociated from human LNs.
(A) FACS plots. The images are representative of six biological replicates.
(B) Comparison of viability of dissociated stromal cells and post-sort LECs. Viability of cells after dissociation were counted by FACS and viability of post-sort cells were counted with Trypan blue. The circles in the Violin plot indicate the number of biological replicates (n=6). ∗p < 0.05, ∗∗∗∗p < 0.0001.