REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
AF488-anti-human CD45 | Biolegend | Cat# 304019; RRID: AB_493033 |
PE-anti-human podoplanin | Biolegend | Cat# 337004; RRID: AB_1595457 |
APC-anti-human CD31 | Biolegend | Cat# 303115; RRID:AB_1877152 |
Chemicals, Peptides, and Recombinant Proteins | ||
RPMI | Sigma | Cat# R5886 |
Phenol-red free DMEM | Sigma | Cat# D1145 |
FCS | Sigma | Cat# F7524 |
HEPES | Lonza | Cat# BE17-737E |
Dispase | Gibco | Cat# 17105-041 |
Collagenase P | Roche | Cat# 11213857001 |
DNase | Roche | Cat# 10104159001 |
DMEM | Sigma | Cat# D6429 |
Critical Commercial Assays | ||
EasySep human CD45 depletion kit | Stem Cell Technology | Cat# 17898 |
LIVE/DEAD Fixable Near-IR Dead Cell Stain kit | Thermo Fisher Scientific | Cat# L34975 |
Other | ||
100 μm cell strainer | BD | Cat# 352360 |
Water bath | N/A | N/A |
Scalpels | Swann-Morton | Cat# 0503 |
Wide bore tips | Sartorius | Cat# 791021 |
Cell sorter | Sony | Cat# SH800S |