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. 2020 Jun 3;1(1):100021. doi: 10.1016/j.xpro.2020.100021
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

AF488-anti-human CD45 Biolegend Cat# 304019; RRID: AB_493033
PE-anti-human podoplanin Biolegend Cat# 337004; RRID: AB_1595457
APC-anti-human CD31 Biolegend Cat# 303115; RRID:AB_1877152

Chemicals, Peptides, and Recombinant Proteins

RPMI Sigma Cat# R5886
Phenol-red free DMEM Sigma Cat# D1145
FCS Sigma Cat# F7524
HEPES Lonza Cat# BE17-737E
Dispase Gibco Cat# 17105-041
Collagenase P Roche Cat# 11213857001
DNase Roche Cat# 10104159001
DMEM Sigma Cat# D6429

Critical Commercial Assays

EasySep human CD45 depletion kit Stem Cell Technology Cat# 17898
LIVE/DEAD Fixable Near-IR Dead Cell Stain kit Thermo Fisher Scientific Cat# L34975

Other

100 μm cell strainer BD Cat# 352360
Water bath N/A N/A
Scalpels Swann-Morton Cat# 0503
Wide bore tips Sartorius Cat# 791021
Cell sorter Sony Cat# SH800S