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smiFISH wash buffer
| Reagent | Final Concentration | Volume (mL) |
|---|---|---|
| MilliQ water | n/a | 80 |
| 20× SSC nuclease-free | 1× | 5 |
| Formamide | 15% | 15 |
| Total | n/a | 100 |
Prepare fresh. Formamide is toxic and should be used in a chemical fume hood. The 6-well plate can be wrapped with plastic film to prevent exposure when smiFISH washes are performed.
