| Chemicals, Peptides, and Recombinant Proteins |
|
| Biotin phenol |
Iris Biotech |
Cat# LS-3500 |
| Biotin |
Sigma-Aldrich |
Cat# 1071508 |
| Trolox |
Sigma-Aldrich |
Cat# 238813 |
| 3% H2O2
|
Milipore |
Cat# 88597 |
| Sodium Ascorbate |
Sigma-Aldrich |
Cat# A4034 |
| Protease Inhibitor Cocktail |
Thermo Fisher Scientific |
Cat# A32965 |
| 4× LDS loading dye |
Thermo Fisher Scientific |
Cat# NP0007 |
| FBS (dialyzed) for SILAC |
Gibco |
Cat# 26400-044 |
| DMEM (dialyzed) for SILAC |
Thermo Fisher Scientific |
Cat# 88364 |
| L-arginine (R0) |
Sigma-Aldrich |
Cat# A8094 |
| L-arginine (R10) |
Cambridge Isotope Laboratories |
CNLM-539 |
| L-lysine (K0) |
Sigma-Aldrich |
Cat# L8662 |
| L-lysine (K8) |
Cambridge Isotope Laboratories |
CNLM-291 |
| Streptavidin Sepharose |
GE Healthcare |
Cat# 17-5113-01 |
| Streptavidin Alexa Fluor 568 |
Thermo Fisher Scientific |
Cat# S11226
|
| Paraformaldehyde (32%) |
Electron Microscopy Sciences |
Cat# 100504-858 |
| Bradford assay |
Biorad |
Cat# 5000006 |
| Vectashield Antifade Mounting medium H1000 |
Thermo Fisher Scientific |
Cat# NC9265087 |
|
| Experimental Models: Cell Lines |
|
| MDCK.2 cells |
ATCC |
ATCC CRL-2936 |
|
| Other |
|
| Transwell filter inserts (6.5 mm) |
Corning |
Cat# 3413 |
| Transwell filter inserts (12 mm) |
Corning |
Cat# 3401 |
| Transwell filter inserts (75 mm) |
Corning |
Cat# 3419 |
| Glass coverslips (12 mm, #1.5) |
Electron Microscopy Sciences (EMS) |
Cat# 72230 |