10× PBS, pH 6.9 [1 L]
| Na2HPO4∙2H2O (177.99 g/mol) | 100 mM | 18 g | |
| KH2PO4 (136.1 g/mol) | 18 mM | 2.4 g | |
| KCl (74.55 g/mol) | 27 mM | 2g | |
| NaCl (58.44 g/mol) | 1.4 M | 80 g |
Weigh in salts, add 800 mL MQ water, adjust pH to 6.9 using HCl (if needed), add to 1 L with MQ water.
Autoclave and store at RT.
Note that pH will be 7.3–7.4 at 1×.