1× PBS⁺⁺ pH 7.4 [50 mL]

10× PBS, pH 6.9	1×, pH 7.4	5 mL	Store stock at 20°C–25°C
50 mM MgCl2	0.5 mM	500 μL	Store stock at 20°C–25°C
100 mM CaCl2	1 mM	500 μL	Store stock at 20°C–25°C
MQ water		44 mL

Prepare fresh as Ca₃(PO₄)₂ precipitates if cold.

Store at RT.