RIPA Buffer [30 mL]

1 M Tris pH 8	50 mM	1.5 mL	Store stock at 20°C–25°C
5 M NaCl	150 mM	900 μL	Store stock at 20°C–25°C
0.5 M EDTA	5 mM	300 μL	Store stock at 20°C–25°C
Sodium deoxycholate	0.5%	150 mg
10% SDS	0.1%	300 μL	Store stock at 20°C–25°C
10% Triton X-100	1%	3 mL	Store stock at 20°C–25°C in the dark
MQ water		24 mL

Prepare fresh and store on ice.