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Trained immunity features of macrophages isolated one week after β-glucan injection compared to control. (A–C) Cells were stimulated in vitro with LPS for 24 h before quantifying cytokine release in the supernatants. (D–I) Immune phenotyping was also performed 24 h post LPS stimulation. (J–L) Dot plot illustration of the six markers and their adjunct histograms normalized to mode. *p value < 0.05; ****p value < 0.0001.
