Figure 5.

BTK, NF-κB, and NLRP3 inflammasome are not activated in Xid mice after polymicrobial sepsis. Mice underwent sham-operated or CLP surgery and 24 h later signaling events in the cardiac tissue were assessed. Densitometric analysis of the bands is expressed as relative optical density (O.D.) of (A) phosphorylation of BTK at Tyr²²³ corrected for the corresponding total BTK and normalized using the related sham bands. (B) Phosphorylation of PLCγ at Tyr¹²¹⁷ corrected for the corresponding total PLCγ and normalized using the related sham bands. (C) Phosphorylation of IKKα/β at Ser^176/180 corrected for the corresponding total IKKα/β and normalized using the sham related bands. (D) Phosphorylation of IκBα at Ser^32/36 corrected for the corresponding total IκBα and normalized using the related sham band. (E) NLRP3 activation, corrected against tubulin and normalized using the sham related bands. (F) Pro-caspase-1 against activated caspase-1 and normalized using the sham related bands. The following groups were studied WT sham (n = 4), Xid sham (n = 4), WT-CLP (n = 4), and Xid-CLP (n = 4). Data are expressed as mean ± SEM and analyzed by one-way ANOVA with a Bonferroni post hoc-test. ***P < 0.001 and ****P < 0.0001 vs. WT-CLP.