Skip to main content
. Author manuscript; available in PMC: 2020 Oct 22.
Published in final edited form as: Oncogene. 2012 Feb 27;32(3):296–306. doi: 10.1038/onc.2012.58

Figure 7.

Figure 7.

Mir-1 and miR-200b induces Slug loss and leads to MET, reduced invasion and growth inhibition in human prostate cancer cells. (a) qPCR analysis of mesenchymal and epithelial markers expression in PC3 cells following control miR (con pre), miR-1 (miR-1 pre) or miR-200b (miR-200b pre) precursor lentivirus stable infection or Slug short interfering RNA (siRNA) transfection. Data represent means±s.e.m., n=3. *: vs Control miR (con miR). *P<0.05, **P<0.01 and ***P<0.001. (b) Representative western blots of EMT markers in PC3 cells after miR precursor lentivirus infection or Slug siRNA transfection. (c) Phase-contrast images of PC3 cells following expression of stable miR precursors or Slug siRNA transfection after 7 days. Scale bars represent 100 μm. (d and e) Cellular invasion of PC3 cells infected with miR precursor lentivirus particles (d) or transfected with Slug siRNA after 7 days (e). Cells that invaded the Matrigel-coated transwells after the indicated times were fixed and counted at × 200. Data represent means±s.e.m., n=5. *: vs Con miR. *P<0.05, **P<0.01 and ***P<0.001. (f) In vitro growth rate of PC3 cells stably expressing miR-1 and miR-200b and control miR precursor lentivirus. Data represents means±s.e.m., n=6. *: vs Con miR. ***P<0.001.