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Design of a CRISPRi-dCas9 system for targeted gene repression in M. acetivorans. The CRISPRi-dCas9 plasmid pDL730 and derivatives can either integrate into the chromosome of M. acetivorans or be converted into a replicating plasmid. Expression of the gRNA and dCas9 is induced by growth with methanol (MeOH) and the addition of tetracycline (Tet).
