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Effect of NH4Cl on M. acetivorans growth and nif operon expression. (A) Growth in HS medium with or without NH4Cl with a 75% N2 headspace. (B) Fold change in nifH and nifD transcript abundance in cells grown in medium with or without NH4Cl as determined by qPCR. (C, top) Western blot analysis using NifD-specific antibodies on lysates from M. acetivorans cells grown with or without NH4Cl. (C, bottom) SDS-PAGE of lysates used for Western blot. MW, molecular weight standard.
