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Designed gRNAs for targeted dCas9-mediated gene repression. (A) gRNAs targeting the promoter (gRNA-Pnif) and 5′ end of nifD (gRNA-nifD) of the nif operon. The BRE, TATA, and NrpR operator sequences are boxed. (B) gRNA targeting the 5′ end of nifB. (C) gRNA targeting the 5′ end of nrpR1. Start codons are in bold and underlined. PAM sequences are in red.
