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Relative transcript abundance of genes targeted for dCas9-mediated repression. (A) Fold change in nifB transcript abundance in strain DJL72 versus DJL105. (B) Fold change in nrpR1 transcript abundance in strain DJL72 versus DJL79. Strains were grown in HS medium with 125 mM methanol and with or without NH4Cl. qPCR was performed as described in Materials and Methods. Data are the means from three biological replicates analyzed in duplicate. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.
