FIG 6.

Impact of the three probiotic strains on BMDC maturation. Probiotic strains were coincubated with BMDCs (results from three experiments performed, each with three independent bacterial cultures). BMDCs without stimulation were used as an experimental control, and PAM2CSK4 (a TLR-2 ligand) and CpG-ODN (a TLR-9 ligand) were used as controls. The maturation of BMDCs was evaluated by FACS analysis: MHC-II, CD80, CD86. CD11c was used as a BMDC surface marker. The supernatant was collected, and interleukin-10 was measured by ELISA. Results are expressed as means ± SEM. Data were compared to those for nonstimulated BMDCs by using the Mann-Whitney U test (*, P < 0.05; **, P < 0.01).