TABLE 6.
β-Galactosidase activities of T1 strains at different stages of growtha
| Strain | Relevant genotype | β-Galactosidase activity (nmol/min/OD600) at the following growth stageb
: |
||
|---|---|---|---|---|
| T−1 | T0 | T1 | ||
| T1g | 167 ± 29 | 108 ± 39 | 206 ± 79 | |
| A20-86 | orfA::lacZ | 140 ± 51 | 5,805 ± 158 | 8,660 ± 610 |
| A3-41 | orfB::lacZ | 224 ± 53 | 3,898 ± 35 | 5,941 ± 180 |
| A11-79 | orfC::lacZ | 180 ± 59 | 4,670 ± 1,060 | 7,012 ± 650 |
| A1-20 | orfD::lacZ | 115 ± 24 | 3,905 ± 714 | 5,440 ± 124 |
| A20-86-A | orfA::lacZ abrB::erm | 7,288 ± 498 | 5,888 ± 296 | 7,240 ± 260 |
The strains were grown in 2216 broth, and β-galactosidase levels were measured as described in Materials and Methods.
T−1, OD600 of ∼1; T0, OD600 of ∼3; T1, OD600 of 4 to 5.