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| Genes | Primers (5′–3′) |
|---|---|
| OCT4 | Fw: GCTGGAGCAAAACCCGGAGG |
| Rv: TCGGCCTGTGTATATCCCAGGGTG | |
| SOX17 | Fw: GAGCCAAGGGCGAGTCCCGTA |
| Rv: CCTTCCACGACTTGCCCAGCAT | |
| FOXA2 | Fw: TATGCTGGGAGCGGTG |
| Rv: TGTACGTGTTCATGCCGTTCA | |
| HNF4 | Fw: GGCAATGTGTCAGGGAGGAA |
| Rv: CAGGGATTTCAGGGGCACTT | |
| AFP | Fw: GCAGCCAAAGTGAAGAGG Rv: TGTTGCTGCCTTTGTTTG |
| ALB | Fw: AGACAAATTATGCACAGTTG Rv: TTCCCTTCATCCCGAAGTTC |
