Figure 2.
The titer of IgY against A. actinomycetemcomitans and P. gingivalis (a) and cross-reactivity of IgY (b) determined by ELISA. Briefly, 96-well polystyrene plates were coated with A. actinomycetemcomitans or P. gingivalis and blocked with PBS containing skim milk powder. Gradient dilutions of IgY crude extract were added to the corresponding wells, followed by incubation with HRP- rabbit anti-chicken secondary antibody. The optical density of each well was determined by the chromochromic degree responding to tetramethylbenzidine. When ODsample/ODnegative ≥ 2.1, the maximum dilution multiple of the sample was determined as the IgY titer. The results are the means of 6 replicates.