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. 2020 Oct 8;8:580090. doi: 10.3389/fbioe.2020.580090

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Copyright © 2020 Nugroho, Kleerebezem and Bachmann.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Prolonged observation of L. lactis NCDO712 pre-cultured in CDMpc-lactose 1%, harvested at mid-exponential growth and transferred to the assay medium with lactose 1%. (A) Colony forming units and intact cell count as measured by flow-cytometry. (B) The different cell fractions of the life-dead staining as measured by flow-cytometry. (C) Lactate accumulation derived from fluorescent measurements and HPLC determination show good agreement (R-squared value of 0.94) (dashed lines indicate semi-logarithmic fit). (D) The organic acid production profiles as determined by HPLC were dominated by lactic acid throughout the experiment.