. 2020 Oct 12;96(11):fiaa212. doi: 10.1093/femsec/fiaa212

Table 4.

Most abundant taxa in fresh feces of puppies from 20 to 52 weeks of age fed the inorganic (SeInorg) and the organic (SeOrg) selenium supplemented diets.

	Age (weeks)							Selenium source				Gender
Taxa	20	28	36	44	52	sd	P value	SeInorg	SeOrg	sd	P value	F	M	sd	P value
p_Actinobacteria*	5.87^a	5.56^a,b	5.28^b	5.54^a,b	4.37^c	0.177	<0.001	5.36	5.28	0.138	0.635	5.23	5.41	0.144	0.366
p_Bacteroidetes	6.81^c	8.23^a	7.92^a,b	8.40^a	7.75^b	0.170	<0.001	7.85	7.79	0.108	0.731	7.74	7.90	0.108	0.311
p_Epsilonbacteraeota	1.95^b	3.39^a	3.11^a	2.70^a,b	1.93^b	0.373	0.009	2.62	2.61	0.296	0.970	2.69	2.55	0.301	0.746
p_Firmicutes^†	9.30^a	8.51^b	8.57^b	8.65^b	7.85^c	0.109	<0.001	8.56	8.59	0.069	0.768	8.66	8.49	0.069	0.085
p_Proteobacteria	6.14	6.28	6.00	6.57	5.90	0.215	0.132	6.40	5.95	0.185	0.103	6.09	6.26	0.180	0.502
c_Actinobacteria	4.68	5.02	4.68	4.43	3.98	0.272	0.124	4.41	4.70	0.173	0.261	6.01	5.92	0.303	0.848
c_Alphaproteobacteria	4.58^a	2.21^b	2.22^b	2.26^b	−1.03^c	0.472	<0.001	2.21	1.88	0.299	0.446	4.75	4.70	0.105	0.761
c_Bacilli	5.55^b,c	6.84^a	6.34^a,b	6.03^a,b	5.07^c	0.398	0.008	6.05	5.88	0.309	0.714	6.05	6.17	0.218	0.705
c_Coriobacteriia**	5.60^a	4.84^b	4.66^b	5.08^b	3.46^c	0.165	<0.001	4.89	4.56	0.104	0.033	7.91	7.78	0.071	0.198
c_Gammaproteobacteria	5.81	6.26	5.98	6.56	5.94	0.2636	0.183	6.35	5.87	0.225	0.147	5.60	5.37	0.171	0.344
o_Clostridiales^†	8.49^a	7.78^b	7.80^b	7.96^b	7.21^c	0.112	<0.001	7.85	7.85	0.071	0.971	4.39	4.72	0.172	0.194
o_Selenomonadales	4.45^d	5.53^b,c	6.38^a	6.02^a,b	5.04^dc	0.269	<0.001	5.72	5.25	0.170	0.059	2.15	1.94	0.297	0.629
f_Burkholderiaceae	4.35^c	6.09^a,b	5.66^b	6.43^a	5.71^b	0.240	<0.001	5.67	5.63	0.180	0.882	5.49	5.80	0.161	0.181
f_Eggerthellaceae*	2.33^a	2.62^a	2.25^a	2.48^a	1.02^b	0.284	0.005	2.09	2.19	0.204	0.733	1.86	2.42	0.211	0.090
f_Enterobacteriaceae	6.07^a	3.39^b	2.76^b	2.11^b	0.40^c	0.476	<0.001	2.74	3.15	0.306	0.377	2.74	3.15	0.306	0.361
f_Erysipelotrichaceae	8.55^a	7.08^b	7.23^b	7.43^b	6.40^c	0.207	<0.001	7.19	7.49	0.196	0.304	7.32	7.36	0.183	0.893
f_Lachnospiraceae*	6.36^a	6.39^a	6.25^a	6.42^a	5.43^b	0.127	<0.001	6.19	6.15	0.080	0.747	6.15	6.19	0.080	0.738
f_Muribaculaceae	3.23^b	6.89^a	6.25^a	6.59^a	6.80^a	0.340	<0.001	5.95	5.96	0.265	0.997	5.51	6.40	0.267	0.012
f_Peptostreptococcaceae	8.18^a	7.26^b	7.26^b	7.29^b	6.60^c	0.169	<0.001	7.28	7.36	0.107	0.609	7.39	7.25	0.107	0.377
f_Rhizobiaceae	4.37^a	2.00^b	2.04^b	2.03^b	−1.04^c	0.465	<0.001	2.04	1.72	0.296	0.445	1.97	1.79	0.292	0.671
f_Ruminococcaceae	6.33^a	5.76^b	6.07^a,b	6.43^a	5.15^c	0.160	<0.001	6.11	5.78	0.110	0.040	5.95	5.95	0.113	0.983
f_Succinivibrionaceae	2.29^b	4.09^a	4.70^a	4.31^a	3.79^a	0.328	<0.001	3.70	3.97	0.211	0.396	4.07	3.60	0.209	0.127
f_Veillonellaceae	4.26^c	5.09^b,c	6.06^a	5.47^a,b	4.35^c	0.297	<0.001	5.27	4.82	0.188	0.099	5.12	4.97	0.190	0.588
g_Allisonella*	0.67^c	2.04^a,b	1.46^b,c	2.47^a	1.22^b,c	0.394	0.034	1.98	1.16	0.249	0.027	1.79	1.36	0.253	0.242
g_Allobaculum	4.47^a,b	5.27^a	2.86^c	3.63^b,c	3.43^b,c	0.384	<0.001	3.89	3.97	0.292	0.827	3.68	4.19	0.289	0.189
g_Alloprevotella	5.18^c	6.32^b	6.18^b	7.06^a	6.29^b	0.220	<0.001	6.30	6.11	0.139	0.325	6.18	6.23	0.140	0.812
g_Allorhizobium-Neorhizobium-
-Pararhizobium-Rhizobium	5.49^c	6.91^a,b	6.66^a,b	7.24^a	6.52^b	0.228	<0.001	6.58	6.55	0.144	0.886	6.45	6.68	0.145	0.265
g_Anaerobiospirillum	2.27^b	3.66^a	4.33^a	4.20^a	3.65^a	0.345	0.002	3.46	3.78	0.224	0.345	3.79	3.45	0.219	0.283
g_Anaerofilum	1.22^b	1.13^b	1.69^a,b	2.45^a	1.39^a,b	0.362	0.081	1.63	1.52	0.228	0.733	1.43	1.72	0.233	0.398
g_Angelakisella	4.17^a	1.30^c	1.83^b,c	2.80^b	1.34^c	0.346	<0.001	2.41	2.17	0.220	0.460	2.30	2.27	0.225	0.922
g_Bacteroides	4.94^c	6.64^a,b	6.29^a	6.75^a	5.84^b	0.233	<0.001	6.07	6.12	0.148	0.824	6.09	6.10	0.148	0.953
g_Bifidobacterium	4.64	5.03	4.68	4.42	3.98	0.288	0.169	4.40	4.69	0.183	0.278	4.38	4.71	0.182	0.207
g_Blautia^†	5.73^a	5.59^a	5.66^a	5.83^a	4.74^b	0.172	0.001	5.50	5.52	0.109	0.883	5.58	5.44	0.110	0.402
g_Butyricicoccus	3.84^a	1.97^b,c	1.86^c	2.50^b	0.93^d	0.203	<0.001	2.33	2.12	0.129	0.254	2.18	2.26	0.129	0.648
g_Candidatus Arthromitus	3.05^a	2.18^a,b	2.71^a	1.85^a,b	0.94^b	0.483	0.036	2.32	1.97	0.314	0.464	2.37	1.92	0.307	0.313
g_Catenibacterium	4.49^a	2.38^b	2.48^b	1.47^b	−1.43^c	0.465	<0.001	2.45	1.31	0.321	0.015	2.22	1.54	0.316	0.125
g_Clostridium sensu stricto 1	−1.45^c	3.06^b	3.31^b	3.70^a,b	4.42^a	0.435	<0.001	2.87	2.35	0.279	0.186	2.54	2.68	0.283	0.722
g_Collinsella*	5.53^a	3.99^c	4.35^b,c	4.66^b	2.66^d	0.205	<0.001	4.46	4.01	0.145	0.043	4.35	4.12	0.141	0.263
g_Dubosiella*	2.19^b	3.26^a	3.23^a	1.62^b	1.22^b	0.397	0.001	1.93	2.69	0.288	0.048	2.09	2.52	0.289	0.269
g_Erysipelatoclostridium	4.37^a	3.34^b,c	2.88^c,d	3.64^b	2.42^d	0.205	<0.001	3.44	3.22	0.133	0.268	3.27	3.39	0.145	0.567
g_Escherichia-Shigella	6.07^a	3.39^b	2.75^b	2.11^b	0.40^c	0.480	<0.001	2.74	3.14	0.308	0.390	2.73	3.15	0.308	0.359
g_Faecalibacterium	5.47^a,b	5.33^b,c	5.75^a,b	5.96^a	4.77^c	0.208	0.002	5.53	5.38	0.148	0.507	5.55	5.36	0.149	0.386
g_Fournierella	0.61^c	2.40^a,b	2.63^a,b	3.06^a	2.01^b	0.307	<0.001	2.27	2.01	0.233	0.415	2.31	1.97	0.234	0.310
g_Fusicatenibacter	−0.48^b	1.50^a	1.58^a	1.87^a	1.27^a	0.293	0.001	1.16	1.14	0.186	0.958	1.63	0.67	0.184	<0.001
g_Fusobacterium	7.41	7.91	7.84	7.92	7.59	0.251	0.496	7.74	7.73	0.190	0.975	7.60	7.87	0.188	0.301
g_Helicobacter	1.90^b	3.39^a	3.10^a	2.68^a,b	1.89^b	0.370	0.006	2.62	2.56	0.294	0.899	2.67	2.51	0.299	0.707
g_Holdemanella	4.03^a	2.77^b	2.93^b	3.05^b	1.92^c	0.201	<0.001	3.16	2.72	0.127	0.017	3.05	2.83	0.128	0.219
g_Howardella	−0.52^c	1.19^a,b	0.81^a,b,c	1.65^a	0.10^b,c	0.437	0.012	0.90	0.39	0.277	0.205	0.46	0.83	0.278	0.359
g_Lachnoclostridium	0.47^c	3.20^a	3.00^a	3.25^a	2.02^b	0.279	<0.001	2.51	2.27	0.196	0.341	2.44	2.34	0.210	0.736
g_Lachnospira	−0.44^c	4.39^a	3.44^a,b	3.96^a	2.89^b	0.426	<0.001	3.22	2.48	0.334	0.140	2.58	3.11	0.336	0.286
g_Lachnospiraceae NK4A136 group	1.19^b	2.04^a,b	2.08^a,b	2.91^a	1.35^b	0.353	0.009	1.96	1.86	0.223	0.739	1.77	2.06	0.226	0.378
g_Lactobacillus	5.38^b,c	6.84^a	6.30^a,b	6.05^a,b,c	5.06^c	0.418	0.012	6.05	5.80	0.315	0.573	5.98	5.87	0.309	0.800
g_Megamonas	2.30^c	5.06^a	5.22^a	5.14^a	4.22^b	0.265	<0.001	4.33	4.45	0.169	0.643	4.34	4.44	0.168	0.694
g_Negativibacillus	0.51^c	3.13^a,b	2.00^a,b	3.17^a	1.70^b,c	0.512	0.003	2.23	1.98	0.384	0.669	1.68	2.52	0.383	0.143
g_Parasutterella	2.72^c	6.00^a,b	5.37^b	6.30^a	5.69^a,b	0.394	<0.001	5.18	5.25	0.311	0.889	4.99	5.44	0.294	0.264
g_Phascolarctobacterium	2.78^b	4.48^a	5.14^a	5.13^a	4.34^a	0.363	<0.001	4.57	4.17	0.230	0.228	4.53	4.211	0.232	0.341
g_Peptoclostridium	6.70	6.63	6.70	6.58	5.96	0.227	0.135	6.48	6.55	0.145	0.724	6.61	6.42	0.145	0.342
g_Peptococcus	1.66^c	3.08^a,b	2.60^a,b	3.22^a	2.36^b,c	0.280	0.001	2.64	2.53	0.212	0.730	2.33	2.84	0.198	0.061
g_Peptostreptococcus	4.79	4.74	5.25	5.00	4.57	0.406	0.690	5.00	4.73	0.341	0.563	4.52	5.21	0.335	0.133
g_Prevotella 9	6.26^b	7.16^a	6.89^a,b	7.26^a	6.17^b	0.255	0.009	6.73	6.76	0.163	0.895	6.78	6.71	0.165	0.771
g_Prevotellaceae Ga6A1 group	2.86	3.52	3.83	3.71	2.89	0.384	0.135	3.71	3.01	0.305	0.100	3.54	3.18	0.305	0.392
g_Romboutsia	7.00^a	5.78^b,c	5.59^c	6.29^b	5.47^c	0.274	0.000	5.78	6.28	0.262	0.197	5.81	6.24	0.258	0.259
g_Ruminococcaceae UCG-005	3.02^a,b,c	3.14^a,b	2.10^c	3.87^a	2.69^b,c	0.377	0.015	3.30	2.63	0.295	0.132	2.66	3.27	0.285	0.141
g_Ruminococcaceae UCG-014	4.28^a,b	3.58^b,c	3.76^b,c	4.68^a	3.12^c	0.306	0.008	4.43	3.34	0.197	<0.001	3.63	4.14	0.195	0.079
g_Sutterella	1.02^b	3.02^a	3.97^a	3.93^a	2.77^a	0.428	0.000	2.97	2.91	0.278	0.874	3.21	2.68	0.276	0.193
g_Turicibacter	8.46^a	6.58^b	7.03^b	7.11^b	5.73^c	0.283	<0.001	6.76	7.20	0.255	0.247	7.06	6.90	0.243	0.635
g_Tyzzerella 3	5.36^a	3.58^b	2.53^b,c	1.23^c,d	−0.37^d	0.572	<0.001	2.49	2.44	0.357	0.932	2.75	2.17	0.378	0.325
g_[Eubacterium] brachy group	1.27^c	3.55^a	2.88^a,b	3.67^a	2.56^a,b	0.307	<0.001	2.98	2.60	0.222	0.258	2.48	3.09	0.206	0.040
g_[Ruminococcus] gauvreauii group^†	2.42^a	2.18^a	2.04^a	2.43^a	1.25^b	0.216	0.006	2.16	1.96	0.137	0.311	2.13	1.99	0.137	0.477
g_[Ruminococcus] gnavus group*	3.05^b	3.65^a	3.34^a,b	3.22^b	2.04^c	0.156	<0.001	3.13	2.99	0.123	0.378	2.99	3.12	0.121	0.408
g_[Ruminococcus] torques group*	2.48^c	3.63^a,b	3.59^a,b	3.93^a	3.26^b	0.186	<0.001	3.61	3.15	0.156	0.051	3.31	3.45	0.148	0.498

^a–d

Values in the same row that share a common superscript are not statistically different (P > 0.05).

sd: standard deviation.

Letters from gender designate: F: female; M: male.

Letters before bacterial groups designate taxa: p_: phylum; c_: class; o_: order; f_: family; g_: genus.

**Interaction between selenium source and age was highly statistically significant (P < 0.001), *interaction between selenium source and age was statistically significant (P < 0.05), ^†interaction between selenium source and age tended to be significant (P < 0.1).