Table 7.

Fecal pH and concentration of end-fermentation products (ammonia, mg/g; biogenic amines, µmol/L; lactate, mM; and volatile fatty acids, VFA, µmol/g) of fresh feces from puppies from 20 to 52 weeks of age fed the inorganic (SeInorg) and the organic (SeOrg) selenium supplemented diets.

	Age (weeks)							Selenium source				Gender
	20	28	36	44	52	SEM	P value	SeInorg	SeOrg	SEM	P value	F	M	SEM	P value
pH*	6.30c	6.30c	6.29c	6.58b	6.71a	0.101	<0.001	6.43	6.44	0.075	0.902	6.42	6.45	0.071	0.814
Ammonia-N*	1.18b,c	1.22b,c	1.76a	1.38b	1.03c	0.126	<0.001	1.28	1.37	0.112	0.497	1.31	1.34	0.120	0.863
Putrescine	2451a,b	3406a	3317a	2545b	1811b	319.8	<0.001	2553	2859	205.9	0.201	3234	2178	205.9	<0.001
Cadaverine	2343a,b	2248a,b	2704a	2469a,b	1522b	395.6	0.002	2516	1998	302.7	0.100	3030	1485	302.7	<0.001
Spermidine*	464	417	367	410	379	28.6	0.112	429	386	17.0	0.076	405	409	14.7	0.822
Spermine	361	315	248	261	242	28.2	0.055	298	272	17.6	0.276	283	288	17.6	0.820
Lactate†	8.26a	3.31b	5.09a,b	1.61c	1.74c	0.870	0.001	3.25	4.75	0.676	0.084	4.28	3.73	0.614	0.385
Total VFA	199a	178a	176a	114b	126b	9.7	<0.001	155	164	5.9	<0.001	162	157	7.1	0.550
Acetate	122a	97.3b	102a,b	58.5c	61.7c	3.21	<0.001	87.7	89.0	2.80	0.690	91.5	85.2	2.82	0.060
Propionate	46.5a	45.5a	46.7a	30.4b	31.8b	2.65	<0.001	36.8	43.5	2.83	<0.001	41.4	38.9	3.66	0.586
Butyrate	18.3b,c	27.1a	19.9b	15.5c	21.7a,b	3.16	<0.001	18.0	23.1	2.37	0.001	21.5	19.6	2.95	0.615
Iso-butyrate	2.71	2.42	2.92	2.66	2.70	0.308	0.356	2.89	2.48	0.222	<0.001	2.86	2.51	0.259	0.203
Iso-valerate†	5.82	4.03	4.90	3.73	3.75	0.430	0.107	4.43	4.46	0.335	0.931	4.90	4.00	0.329	0.020
Valerate**	2.01a,b	0.80c	0.81c	1.84b	2.77a	0.228	<0.001	1.51	1.79	0.202	0.334	1.69	1.60	0.163	0.582
Iso-caproate*	0.61b	0.62b	0.66b	1.02a	1.22a	0.187	0.002	0.82	0.84	0.188	0.916	0.56	1.10	0.202	0.038
Caproate	1.39a	0.55b	0.54b,c	0.42c	0.36d	0.058	<0.001	0.65	0.65	0.039	0.722	0.70	0.60	0.040	<0.001
Heptanoate†	0.12a	0.06b	0.06b	0.05b	0.06b	0.009	<0.001	0.07	0.07	0.005	0.995	0.07	0.07	0.005	0.574

SEM: standard error of the mean.

Letters from gender designate: F: female; M: male.

^a–cValues in the same row that share a common superscript are not statistically different (P > 0.05).

**interaction between selenium source and age was highly statistically significant (P < 0.001), *interaction between selenium source and age was statistically significant (P < 0.05), ^†interaction between selenium source and age tended to be significant (P < 0.1).