Table 2. High impact singletons in intolerant orthologs for unrelated cats with disease traits.

SNV locationa	Ref/Alt	Consequence	Gene symbol	pLI	Individual ID	Disease	Status
chrA1:116653102	G/A	Stop gained	FAM13B	0.92	felCat.Fcat19194.Pudge	Ectodermal dysplasiab	Affected
chrA1:192824838	G/A	Stop gained	CYFIP2	1.00	felCat.Fcat20406.Gannon	Stones	Affected
chrB1:77305060	C/T	Stop gained	FBXW7	1.00	felCat.Fcat5012.Coloradoc	Lymphoma	Carrier
chrD2:63980875	G/A	Stop gained	SH3PXD2A	1.00	felCat.CR1397.Isabella	Infectious peritonitisd	Affected

^a SNV locations were only reported if they were supported by NCBI annotations.

^b A second unrelated cat, felCat.Fcat19197.Kooki, was also affected.

^c Affected offspring removed earlier from analysis inherited the same SNV.

^d A second unrelated cat, felCat.CR1219.Tamborine, was also affected.