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. 2020 Oct 12;14(10):e0008750. doi: 10.1371/journal.pntd.0008750

Fig 1. Sensitivity comparison of conventional PCR and qPCR for HSP70 target.

Fig 1

(A) Electrophoresis results of cPCR for L. (V.) braziliensis and L. (V.) guyanensis: 1: ladder 100 pb; 2: Negative control; 3 to 10: serial dilution from 104 to 10−3 par. eq./reaction of L. (V.) braziliensis, respectively; 11: Negative control; 10 to 19: serial dilution from 104 to 10−3 par. eq./reaction of L. (V.) guyanensis, respectively. (B) Electrophoresis results of cPCR for L. (L.) amazonensis and L. (V.) panamensis. 21: Negative control; 22 to 29: serial dilution from 104 to 10−3 par. eq./reaction of L. (L.) amazonensis respectively; 30: Negative control; 31 to 38: serial dilution from 104 to 10−3 par. eq./reaction of L. (V.) panamensis respectively. (C) Ct values of qPCR reaction with HSP70 target for L. (V.) braziliensis, L. (V.) guyanensis, L. (L.) amazonensis, L.(V.) panamensis. OBS: The same serial dilutions from 104 to 10−3 Par. Eq./reaction, used in cPCR, were used in qPCR reactions, to determinate the dynamic extension of detection.