Table 5. Sensitivity and specificity of Leishmania detection by real time PCR (qPCR) in comparison to microscopy.
| Assays | Patient samples (n = 88) | |
|---|---|---|
| Microscopy + | Microscopy - | |
| qPCR HSP70 + | 46 (83.6%) | 13 (39.4%) |
| qPCR HSP70 – | 9 (16.4%) | 20 (60.6%) |
| Total | 55 | 33 |
| qPCR 18S rDNA + | 50 (90.9%) | 27 (81.8%) |
| qPCR 18S rDNA– | 5 (9.1%) | 6 (18.2%) |
| Total | 55 | 33 |
Sensitivity and specificity values for qPCR assays using the 18S rDNA and HSP70 targets in comparison to microscopy were obtained for the 88 patient samples analyzed.