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. 2020 Oct 22;54:101669. doi: 10.1016/j.mcp.2020.101669

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Fig. 1 — FCoV-RPA assays. (A) The screening of the primer sets. (B) Sensitivity assessment of the FCoV-RPA assay was performed with 10⁶–10¹ copies/μL DNA standard dilutions. 6: 10⁶ copies/μL, 5: 10⁵ copies/μL, 4: 10⁴ copies/μL, 3: 10³ copies/μL, 2: 10² copies/μL, 1: 10¹ copies/μL, 0: indicates negative control. (C) Analytical sensitivity of FCoV-RPA assay (triangle). (D) Specificity of the FCoV-RPA assay was observed. Nucleic acids of FCoV, CDV, CCoV, CAV, FHV, FPV, and FCV were tested to assess specificity.