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The day-to-day and within-day variations of the RPA assay. Data listed in this table were from three independent RPA reactions using 106 copies/μL of standard plasmid.
| Within-day variation | Day-to-day variation | ||||
|---|---|---|---|---|---|
| TT | mean | CV% | TT | mean | CV% |
| 6:00 | 6:06 | 2.5% | 5:90 | 6:11 | 3.0% |
| 6:23 | 6:18 | ||||
| 5:95 | 6:24 | ||||
CV: coefficient of variation; RPA: recombinase polymerase amplification; TT: threshold time.
