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. 2020 Oct 12;16(10):e1008956. doi: 10.1371/journal.ppat.1008956

Fig 1. AELRP sequence in HCPro is predicted to bind to proteins containing a WD40-domain.

Fig 1

(A) Percentage of amino acid conservation within the AELPR motif in 119 potyviral HCPro sequences. This motif was identified by scanning the PVA HCPro sequence for short linear motifs (SLiMs) via ELM server (http://elm.eu.org/). The AELPR motif was predicted to be a putative WD40 -domain-interacting motif. (B) Ribbon diagram of X-ray crystallographic structure of the cysteine protease domain of TuMV HCPro (PDB ID: 3RNV). Secondary structure of the ‘AELPR’ motif has been highlighted in red. (C) Surface diagram of 3RNV showing the accessibility of the WD40-domain -interacting motif in HCPro (highlighted in red). (D—F) Schematic representation of the constructs prepared for this study (not in scale): (D) HCPro overexpression constructs. (E) icDNAs of the full length PVA constructs. (F) VCS overexpression constructs.