Figure 2.

Lung Pathology

(A–E) Photographs (scale graduations, mm) of freshly harvested lungs after the first (A), second (B), third (C), and fourth (D) doses show progressive reduction in nodular lesions, while the lungs of mice receiving no treatment showed a large number of lesions (E). Photographs were subjected to image analysis using ImageJ software. (F) The numbers of nodular lesions (scatter points) reduced progressively, and their dimensions were more diffuse in animals receiving inhalations. Scatter points refer to individual lesions. The average number of lesions at each time point/group is shown as a line, with error bars showing standard deviations. Statistically significant differences (t test, α = 0.05, actual power 0.67) are shown by asterisks. NS, not significant.